Epigenetic changes in Langerhans islets preceding the onset of diabetes

Diabetes-resistant and diabetes-prone female New Zealand Obese mice were classified based on liver fat content and early blood glucose concentrations at 10 weeks of age before the onset of T2D. By using transcriptome and DNA methylome analysis of Langerhans islets, we identified early epigenetic alteration in mice and humans which could serve as putative epigenetic biomarkers Overall design: For Bisulfite seq, we used 5 samples per group for diabtes prone (DP) and resistant (DR). RNA-seq was performed with 4 DR and 5 DR. In our study design, DR animals are healthy animals and are considered as control group