Genome-wide maps of chromatin openness in control and LMNA R527C mutant MSCs. Genome-wide maps of chromatin openness in control and LMNA R527C mutant MSCs

We performed ATAC-seq to examine to chromatin of openness of coding genes in healthy control and LMNA R527C patient-derived MSCs. The reduction of LADs did not increase the accessibility of the promoters flanking coding genes. The genome wide analysis of ATAC-seq showed that openness of coding genes related chromatin was decreased in mutant MSCs.Thus, R527C mutant Lamin A led to dysfunction of LADs and dysregulation of gene transcription, probably, leading to slow down or arrest of cell proliferation and differentiation. Overall design: LADs were distributed across heterochromatic, promoter, and actively transcribed regions of the genome and were associated with DNA methylation and suppressed gene expression. We thus performed ATAC-seq to examine to chromatin of openness of coding genes in control and LMNA R527C mutant MSCs as Lamin A-LADS binding affinity were changed during LMNA R527C mutation. Examination of chromatin openness in control and LMNA R527C mutant MSCs.