Effect of organic fertilizer substitution on nitrogen metabolism of highland barley

To investigate the effects of organic fertilizer replacing chemical fertilizer on the growth and development of barley (Kunlun-14), a pot experiment was conducted. The study examined the impacts of different ratios of organic fertilizer replacing chemical fertilizer nitrogen (0%, 40%, 100%, denoted as OFR0, OFR40, OFR100, respectively) on the growth characteristics, leaf carbon-nitrogen balance, and nitrogen metabolism enzyme activities of barley. Overall design: Design three different proportions of organic fertilizers replacing chemical fertilizer nitrogen (0%, 40%, 100%), recorded as ORF0, ORF40, and ORF100 respectively. The total application amounts of N, P, and K in each treatment should be kept consistent. In the pot experiment, each flower pot is calculated based on 3.5kg of soil. ORF0 uses urea: 357mg, superphosphate 1545mg, and potassium sulfate 140mg; ORF40 uses urea: 215mg, superphosphate 1443mg, potassium sulfate 122mg, and organic fertilizer 1117mg; ORF100 uses superphosphate. 1289 mg, potassium sulfate 96 mg, and organic fertilizer 2793 mg; the above-ground and underground parts of highland barley were collected during the flowering period for transcriptome sequencing.