Dnmt1 deletion in PV-positive cortical interneurons of 6 month and 18 month old mice [RNA-seq]

The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed RNA-Seq. 8 samples in total; WT and KO condition of 6 month and 18 month old mice, each in 2 technical replicates; cells were pooled from 6 individual wild type 6 individual knockout mice. Per mice both hemispheres were FAC-sorted independently and the cells from one hemisphere were used for RNA extraction while the other one were used for DNA extraction.