Expression data from mouse aorta with chronic intermittent hypoxia exposure

Atherosclerosis is the pathological basis of cardiovascular disease. Obstructive sleep apnea aggravates atherosclerosis, and chronic intermittent hypoxia (CIH) as a prominent feature of obstructive sleep apnea plays an important role during the process of atherosclerosis. The mechanisms of CIH in the development of atherosclerosis remain unclear. The microarray was used to investigate differentially expressed mRNAs and long noncoding RNAs (lncRNAs) in aorta from five groups of ApoE-/- mice fed with a high-fat diet and exposed to various conditions: normoxia for 8 weeks, CIH for 8 weeks, normoxia for 12 weeks, CIH for 12 weeks, or CIH for 8 weeks followed by normoxia for 4 weeks. For microarray profiling, the mouse aorta total RNA was harvested using TRIzol reagent (Life Technologies, CA, USA) and purified using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The amount and quality of RNA were determined using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, MA, USA). The mRNA expression profiling was measured using Affymetrix ClariomTM D mouse Array (Affymetrix, Thermo Fisher Scientific), which contains 65956 gene-level probe sets.