Summary of the culture conditions tested and the best culture conditions in each case.

Data are shown as mean ± SEM for sample size n.
The best culture conditions were determined in experiments 1–7, and then a conclusive experiment 8 was performed.
E18, E19: Primary neurons isolated from rat fetal hippocampi on embryonic days 18 and 19.
*medium change (+): medium changes.
*medium change (–): no medium changes.
*For medium name, see “Culture medium” section.