MeRIP-seq to explore the targets of METTL3 in pancreatic cancer
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https://www.ncbi.nlm.nih.gov/sra/SRP349091
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We performed MeRIP-seq of METTL3-knockdown (KD) MIAPaCa-2 and control cells to detect METTL3-methylated genes and extracted genes whose methylation was lost in METTL3-KD cells compared with control cells. Next, we performed mRNA expression analysis using dates of input samples at MeRIP-seq. Genes whose methylation peaks were abolished by METTL3-KD and whose expression changes were observed were analyzed as candidates for METTL3-regulated genes. Overall design: RIP-sequence data using m6A Ab
创建时间:
2022-07-08



