Analysis of the EGFR gene in the RA2 ERL-resistant cell line.

A) Analysis of EGFR exon 19 nucleotides sequence. The pherogram of the parental cell line with peaks corresponding to the EGFR mutated sequence (ΔE746-A750) and the pherogram of the RA2 resistant cells with peaks corresponding to the mutated and wild type (WT) EGFR nucleotides sequence are shown. B) qPCR analysis. Relative EGFR gene copy number (GCN) in genomic DNA, normalized to the Rnase P gene, is expressed relative to the levels in parental cell lines (P) set as 1 (mean ± SD of triplicate determinations). Results are representative of those obtained from 2 independent analysis.