Expression data from day of isolation and day 4 suspension cultured human pancreatic exocrine cells

Donor pancreata were obtained from the Beta Cell Bank of the JDRF Centre for Beta Cell Therapy in Diabetes (Brussels, Belgium), from Pancreatic Islet Processing (ECIT center) of Diabetes Research Institute at the IRCCS San Raffaele Scientific Institute (Milan, Italy) and from the DRWF Human Islet Isolation Facility (Oxford, England). Full written consent for use of donor material for research was obtained according to Belgian, Italian and English laws. This project was approved by the Medical Ethical Committee of all institutions. Cells were cultured in 3D suspension culture for four days in Advanced RPMI supplemented with 5% FBS. We used microarray to determine temporal changes in transcriptome of human panreatic exocrine cells at day of isolation and day 4 of suspension culture RNA of culster was extracted using RNeasy micro kit (Qiagen; 74004)