Regulation of gene expression by Ankrd35 in LKR10 cells grown in 2D monoculture or as 3D clusters in methylcellulose

The objective of this study was to use RNAseq to determine which gene change in expression when LKR10 cells are grown in monolayers (2D) or methylcellulose on ultra low attachment plates (3D), which prevents attachment of cells to the plate and promotes formation of 3D sphere-like clusters. A secondary objective was to determine whether knockdown of Ankrd35 altered gene expression of specific genes in 2D or 3D. Overall design: Examination of gene expression in the murine LKR10 cell line with and without knockdown of Ankrd35 in traditional 2D monolayer cultures and in 3D methylcellulose cultures by RNAseq