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Total RNA-seq of Day-21 WT and MTR4-null nucleus and cytoplasm

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NIAID Data Ecosystem2026-05-01 收录
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Mtr4 deletion caused unbalanced mRNA distribution between different subcellular components like nucleus and cytoplasm. In order to obtain a global view of the abnormally distributed transcripts, total RNA-seq of nucleus and cytoplasm seperated from Day-21 WT and MTR4-null oocytes were conducted in this study. Total RNA were extracted by TRIzol. rRNA were removed. 10 nuclei or cytoplasms were used per sample.

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2023-09-12
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