The RNA-binding protein hnRNPF is essential for germinal center reaction

Alternative splicing (AS) plays an important role in the development and activation of B cells. RNA-binding proteins (RBPs) have emerged as critical players in AS regulation. RNA-binding protein hnRNPF regulates AS of several important genes, such as c-Src, Bcl-xl, Mcl-1 and Tcf3 in tumor cells, but its function in B cell biology is not known.Here we constructed CD19-Cre mediated hnRNPF conditional knockout mice and performed RNA-sequencing to profile mRNA expression and AS regulated by hnRNPF in splenic B cells. RNA sequencing of splenic follicular B cells in wild-type (WT) and hnRNPF knockout (HFKO) mice