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Gene expression profiling of human iPSC kidney organoid-derived epithelium

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NIAID Data Ecosystem2026-04-25 收录
下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP125589
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The kidney organoid differentiation protocol takes induced pluripotent stem cells through to kidney organoid via directed differentiation in approximately 25 days. The cells are grown in a monolayer in a dish for seven days and are subjected to growth factors before being pelleted on day seven. The organoids then continue to differentiate as a 3D structure, with at least 8 distinct kidney cell types identifiable around day 18. Here the EPCAM+ epithelial fraction was isolated from day 25 kidney organoids using MACS-enrichment and RNA-sequencing libraries generated. Overall design: RNA-Seq of three replicates of EPCAM+ epithelial fraction isolated from human kidney organoids
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2019-09-23
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