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DataCite Commons2020-08-25 更新2024-07-28 收录
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U87 human cells were infected with GFP+ Venezuelan Equine Encephalitis Virus (VEEV) strain TC-83 with 3 MOIs (0.1, 1, 3) and harvested at 6 time points (0.5 - 24 hours post infection). viscRNA-Seq was performed as in Zanini F, Pu SY, et al. eLife (2018).<br>The file is a zip archive containing two files:- counts table with the gene expression of human genes, VEEVGFP, ERCC spike-in RNA controls, and other features from the aligner (e.g. unmapped reads). The counts are unnormalized, raw read pair numbers.- cell_metadata table with the following columns: - coverage: total number of uniquely mapped human reads - VEEV_counts: total number of viral reads - plate: microtiter plate each cell was sorted into (to spot batch effects) - well: the well within each plate each cell was sorted into - time [h]: time post infection - vRNA_5' [cpm]: viral reads in bases 1-1700 of the viral genome, normalized by human coverage per million reads - vRNA_3' [cpm]: viral reads in bases 7501+ of the viral genome, normalized by human coverage per million reads
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figshare
创建时间:
2020-02-19
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