eNampt, CRH and Il-6 influence on gene expression of rat primary corticotropes.. eNampt, CRH and Il-6 influence on gene expression of rat primary corticotropes.

ACTH is known to be secreted from pituitary cortictropes after CRH and Il-6 stimulation. Recenetly it was proven that eNampt also stimulates ACTH secretion from rat pituitary gland. We used microarrays to detail the global programme of gene expression in isolated rat pituitary corticotropes, 24h after administration of eNampt, CRH and Il-6. Overall design: Rat corticotropes were isolated from primary rat pituitary gland culture with anti-CRH antibody used as selection marker. Isolated Rat cortictropes were subjected to the cell culture. Subsequently eNampt, CRH and Il-6 were administered into the cell medium. After 24h, RNA were extracted and hybridize into the Affymetrix microarrays.