Effects of GrpE-DnaK-DnaJ system mutations on Red-mediated recombination.

a. Numbers represent the ratios of recombinants to transformants, normalized to the ratio observed for the wild type strain in the same experiment. Means and standard errors for three experiments are shown. The absolute ratio of chloramphenicol-resistant recombinants (dsDNA cassette) to kanamycin-resistant transformants for wild type had a mean value of 0.078 and a standard deviation of 0.038 in 11 experiments; the corresponding figures for streptomycin-resistant recombinants (oligonucleotide) were 0.25 and 0.08, respectively.b. Numbers represent numbers of recombinants recovered, normalized to the number recovered with a wild type recipient in the same experiment. Means and standard errors for three experiments are shown.c. All strains bear the recBCDΔred substitution, except for TP1625, in which recBCD is deleted without any substitution.d. nrd = no recombinants detected.