Androgen receptor Serine 81 phosphorylation is coupled to AR-mediated transactivation and co-sustained by CDK1 and CDK9 in prostate cancer

We examine total AR verus pSer81-phosphorylated AR chromatin-binding profiling in DHT-treated LNCaP cells to address the molecular functions of pS81 on the chromatin, and demonstrate that S81 phosphorylation can be mediated by both CDK1 and CDK9 at basal and androgen-stimulated levels. 2 total AR and 3 pSer81-AR samples based on DHT-treated LNCaP cells are subjected to ChIP-Seq analyses