Richard Wheeler (2010) CIL:10601, Leishmania mexicana, parasite. CIL. Dataset

Single Leishmania mexicana cell during late mitosis showing the dividing nucleus and kinetoplast (blue DAPI channel) and the sub-pellicular microtubule array and spindle microtubule arrangement (green KMX-1 channel). Promastigote form Leishmania mexicana (WHO strain MNYC/BZ/62/M379) were grown in M199 (10% FCS, pH 7.4, 28°C). Fixed with 2% paraformaldehyde. DNA labelled with 4',6-diamidino-2-phenylindole (DAPI) and tubulin lebelled with KMX-1 anti-beta tubulin monoclonal antibody (Birkett et al. 1985 FEBS Lett. Aug 5;187(2):211-8. PMID: 4018260). Z stack of 35 images with a z spacing of 0.194 um captured with a 100x/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu). Note there is also a phase channel image that is not currently displayed, but can be turned on in the detailed viewer.

单一利什曼原虫梅斯卡纳细胞在晚有丝分裂阶段的图像，展示分裂的细胞核及动基体（蓝色DAPI通道）以及亚质膜微管阵列及纺锤体微管排列（绿色KMX-1通道）。利什曼原虫梅斯卡纳原虫形式（世界卫生组织菌株MNYC/BZ/62/M379）在M199培养基中培养（含10%胎牛血清，pH 7.4，28°C）。用2%多聚甲醛固定。DNA用4',6-二氨基-2-苯基吲哚（DAPI）标记，微管蛋白用KMX-1抗β-微管蛋白单克隆抗体（Birkett等，1985年8月5日《生物化学杂志通讯》第187卷第2期：211-8. PMID: 4018260）标记。使用Leica DM5500 B共聚焦荧光显微镜配100倍/1.4 NA物镜，以及Hamamatsu冷却型CCD相机，捕获了35张图像的Z堆栈，Z间距为0.194微米。注意，还存在一个相位通道图像，目前未显示，但在详细查看器中可以打开。