This study was undertaken to verify whether treatment of human airway epithelial Calu-3 cells with peptide nucleic acids targeting miR-101-3p and miR-145-5p alters the miRNome.

The data show that peptide-nucleic acids (PNAs) inhibiting miR-101-3p and miR-145-5p, two microRNAs targeting the 3'UTR of the Cystic Fibrosis (CF) Transmembrane Conductance Regulator (CFTR) mRNA, have differential effects on the miRNome. While the expression of most microRNAs is not affected by PNA treatment, some of them are strongly modulated. In particular some microRNAs involved in CF and/or CFTR regulation are co-inhibited by miR-101-3p and miR-145-5p. Among them, miR-155-5p, miR-125b-5p, miR-132-3p and miR-6873-3p.