Transcriptional profiling of Primary Human Hepatocytes (PHH) depleted for CAR and treated with CITCO or Phenobarbital in -/+EGF medium. Homo sapiens

In primary human hepatocytes (PHH) the involvement of the Constitutive Androstane Receptor (CAR) in genes regulations has never been evaluated at the transcriptomic level. Here we investigated the impact of CAR depletion and epidermal growth factor on CAR dependent gene modulation in PHH. The xenosensor was activated by its direct (CITCO) or indirect (Phenobarbital/PB) agonists in presence or in absence of EGF. Overall design: Primary Human Hepatocytes were isolated from 5 different human donors. Cells were cultured in presence or in absence of EGF for 6 days. Transfections with siRNA unspecific (siCT) or targeting CAR (siCAR) were performed at day 2 and 5. RNA was extracted at D6 after 24h treatment with DMSO, CITCO (1µM) or PB (0.5mM). Transcriptomic profiling was performed using Affymetrix HG-U133+PM arrays.