Transcriptomic exploration of rutin's regulation of PPARγ inhibition of adipogenic differentiation in 3T3-L1 cells

AimTo explore the mechanism of rutin regulating the peroxisome proliferator activated receptor gamma (PPARγ/PPARG) pathway to inhibit adipogenic differentiation of 3T3-L1 preadipocytes based on transcriptomics.Methods3T3-L1 preadipocytes were cultured in vitro and treated with 25, 50, and 100 μmol·L-1 rutin during the induction of differentiation into mature adipocytes; at the same time, a treatment group was set up with 100 μmol·L-1 rutin and PPARγ activator rosiglitazone. Oil red O staining was used to observe the accumulation of lipid droplets; RNA seq transcriptomics was employed to detect mRNA differential expression; RT-qPCR technology was used to detect the expression of PPARγ mRNA; Western blot was used to detect the expression of PPARγ and other proteins.ResultsRutin at concentrations of 25, 50, and 100 μmol·L-1 exhibited dosedependent inhibition of lipid accumulation (P P -1 rutin group, and the analysis results speculated that PPARγ may play a key role in them. Experimental verification showed that 50 and 100 μmol·L-1 rutin reduced the expression levels of PPARγ mRNA and protein (P -1 rutin dose dependently reduced the expression of PPARγ and its downstream gene proteins (P P P -1 rutin and rosiglitazone partially reversed the PPARγ pathway inhibited by 100 μmol·L-1 rutin, resulting in upregulation of protein expression (P P ConclusionRutin significantly inhibits adipogenic differentiation of 3T3-L1 cells by suppressing PPARγ expression, which lays a theoretical foundation for obesity treatment related research.