Projects related to coordination, the reduction per study and estimated annual reduction.

NA = Not analyseda) Not possible to estimate as the number of studies varies between years. To be evaluated after 2–3 years.b) Not possible to estimate as the number of investigative studies varies between years. Probably an annual reduction of more than hundred rats. To be evaluated after 2–3 years.c) Not possible to estimate as the number of method development and/or validation studies varies between years. Probably an annual reduction of more than one hundred rats. To be evaluated after 2–3 years.d) Not possible to estimate as the number of biomarker studies varies depending on present drug projects and safety assessment issues. Probably an annual reduction of more than one hundred rats. To be evaluated after 2–3 years.e) Not possible to estimate as the projects vary in nature.f) Risk for too low exposure for micronucleus evaluation is addressed and during scientifically investigation and validation before implementation as default.