IRF2BP2 affects T-ALL cell proliferation by cooperating with master transcription factors [Cut&Tag]

Knocking down IRF2BP2 led to decreased T-cell acute lymphoblastic leukemia (T-ALL) cell survival and growth both in vitro and in vivo. To explore IRF2BP2-dependent gene regulation, RNA-seq analysis was conducted and the differently expressed genes were revealed in the IRF2BP2-knockdown J.gamma1 cells in comparison to control group. Cleavage Under Targets and Tagmentation (CUT&Tag) showed the co-localization of IRF2BP2 with several master transcription factors on chromatin in J.gamma1 cells. CUT&Tag using antibody against IRF2BP2, RUNX1, ERG, ELF1 and ETS1 in J.gamma1 cells.