Working scheme for CMPK folding- and unfolding pathways.

Folding scheme for folding and refolding of CMPK, omitting refolding from the Uc species. The x-axis represents the reaction coordinate, while the y-axis corresponds to fluorescence intensity of Trp31 during refolding of wildtype CMPK. Each horizontal line indicates a macroscopic species, as observed in the folding experiments. The native (Nc) and unfolded state with trans-Pro124 (Ut) are indicated separately. Vertical double arrows represent burst-phases. Arrows heading left indicate folding transitions, arrows heading right unfolding transitions. The transition rate constants corresponding to each arrow are positioned next to it. The dotted line indicates the energy barrier associated with the slow folding and unfolding process, separating the cis- from the trans- Pro124 species.