Defining the role of the miR-145 – KLF4 – αSMA axis in mitral valvular interstitial cell activation in myxomatous mitral valve prolapse using the canine model

Mitral valve prolapse (MVP) is a common valvular disease, affecting 2-3% of the adult human population and is a degenerative condition. 5-10% of the afflicted will develop severe mitral regurgitation, cardiac dysfunction, congestive heart failure, and sudden cardiac death. Naturally occurring myxomatous MVP in dogs closely resembles MVP in humans structurally, and functional consequences are similar. In both species, valvular interstitial cells (VICs) in affected valves exhibit phenotype consistent with activated myofibroblasts with increased αSMA expression. Using VICs collected from normal and MVP-affected valves of dogs, we analyzed the miRNA expression profile of the cells and their associated small extracellular vesicles (sEV) using RNA sequencing to understand the role of non-coding RNAs and sEV in MVP pathogenesis. miR-145 was shown to be upregulated in both the affected VICs and sEV, and overexpression of miR-145 by mimic transfection in quiescent VIC recapitulates the activated..., miRNA and RNA sequencing analyzed by Qiagen Genomic Workbench CLC and DESeq2. Western blot and PCR., , * Title of Dataset: Data for the article \"Defining the role of the miR-145 – KLF4 – αSMA axis in mitral valvular interstitial cell activation in myxomatous mitral valve prolapse using the canine model\" * Persistent Identifier: * Dataset Contributors: Vicky K. Yang, Nicole Moyer, Runzi Zhou, Sally Carnevale, Dawn M. Meola, Sally R. Robinson, Guoping Li, Saumya Das * Contact Information: Name: Vicky K. Yang Affiliation: Department of Clinics Sciences, Cummings School of Veterinary Medicine, Tufts University Email: --- * This data includes four files with information on canine mitral valve miRNA, mRNA, PCR, and protein information in association with the developement of mitral valve prolapse. * Description of File 1: File name: PCR Western Luciferase raw data.xlsx Data structure: Data are organized based on figures. Each dataset is associated with a particular figure (worksheet name). General data description: This file contains data associated wi...