Nanobioreactor Detection of Space-Associated Hematopoietic Stem and Progenitor Cell Aging

This study examined the effects of exposure to low-Earth orbit (LEO) on hematopoietic stem and progenitor cell function, gene expression, telomere length, mutation burden, and ADAR1 isoform expression, evaluated 32-45 days post-spaceflight compared to ground control in 3D nanobioreactors. Enriched CD34+ hematopoietic stem and progenitor cells (HSPCs) derived from human normal bone marrow were transduced with a FUCCI2BL cell cycle reporter, and seeded into twin niche nanobioreactors for month-long culture on either the International Space Station (spaceflight) or at Kennedy Space Center (ground control) as part of NASA SpaceX Commercial Resupply Service missions (SpX-CRS24, SpX-CRS25, SpX-CRS26, and SpX-CRS27). Upon mission completion, live cells were flushed from the nanobioreactors and... (for more see dbGaP study page.)