Expression data from TCF19 knockdown in HepG2 cells maintained under high glucose (40mM) condition

Changes in concentration of glucose in the cellular environment results in a variety of changes in the transcription program. Liver is the primary organ for metabolic regulation in the body, and hence, any surge in circulating blood glucose leads to changes in transcriptional states of important enzymes tasked to maintain metabolic homeostasis. Chromatin modification reader proteins play an important role in maintaining metabolic homeostasis by identification of the histone modification, and facilitating recruitment of chromatin remodelling enzymes. We knocked down an important reader domain containing protein, TCF19, in human hepatocellular carcinoma cells ( HepG2) maintained under high glucose condition and used microarray to investigate the changes in gene expression profile. Gene expression profile was compared between two experimental sets : Control ( HepG2 cells treated with non-targeting siRNA and maintained in 40mM glucose for 48 hours) vs. TCF19 siRNA treated ( HepG2 cells treated with TCF19 siRNA, and maintained in 40mM glucose for the same time period as Control set)