Immunohistochemistry
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https://figshare.com/articles/dataset/Immunohistochemistry/6485174
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资源简介：
enograft
tumor from in vivo experiment was havested and fixed in 4% paraformaldehyde.
Paraffin-embedded sections were used for haematoxylin-eosin (HE) staining and
immunohistochemistry assays. For immunohistochemical staining, the sections
were incubated with first antibody of SP1 (1:200, 9389, Cell Signaling
Technology) or CCR7 (1:200, ab32527, Abcam) at 4℃ overnight. Then incubated in biotinylated Goat anti rabbit IgG at 37℃ for 30min, following by herseradish peroxidase (HRP) labeled avidin
incubation. After visualization with diaminobenzidine (DAB), the sections were
counterstained with hematoxylin and observed under 400x. Negative controls were
obtained by omitting the primary antibodies. The expression of SP1 and CCR7
protein was semi-quantitatively measured by using Image Pro Plus 6.0 (Media
Cybernetics, Inc., MD, USA).
提供机构：
figshare
创建时间：
2019-02-08
