Smarcad1 coordinates innate immunity-linked gene expression in the intestinal epithelium [Cocr RNA-seq]

Smarcad1 is one of the most conserved chromatin remodelling factors, from yeast to human, with suggested functions in gene silencing, heterochromatin maintenance and genome stability. However, its role in tissue function is poorly understood. As this factor is highly expressed in the stem- and proliferative zone in the intestinal epithelium, we explored the role of this factor in this tissue. Intestine tissue-specific deletion of Smarcad1 resulted in notable changes in gene expression in the small intestine, colon and organoids from small intestine. Comparisons between wild type mice and Vil-Cre mediated KO of Smarcad1 in the intestinal epithelium. C57BL/6 background. RNA-seq performed in triplicates on colon epithelial cells (CO) isolated as whole crypts.Smarcad1是从酵母到人类中最为保守的染色质重塑因子（chromatin remodelling factor）之一，其功能被推测参与基因沉默、异染色质维持与基因组稳定性调控。然而，学界对其在组织功能中的作用仍缺乏深入认知。鉴于该因子在肠上皮的干细胞区与增殖区中呈高表达，我们针对其在该组织中的功能开展了相关研究。对肠道组织特异性敲除Smarcad1后，小肠、结肠以及小肠类器官的基因表达均出现显著变化。本实验采用C57BL/6遗传背景小鼠，对比了肠上皮中经Vil-Cre介导的Smarcad1基因敲除（Knock Out, KO）小鼠与野生型小鼠的差异；并以完整隐窝分离得到的结肠上皮细胞（CO）为样本，完成了三次生物学重复的RNA测序（RNA sequencing, RNA-seq）。