Measurements of C–P lyase phosphate thresholds in Stutzerimonas frequens cultures (North Pacific Methane Project)

This dataset contains results from an experiment investigating the phosphate concentration threshold for C-P lyase activity in cultures of Stutzerimonas frequens. The dataset includes measurements of total dissolved phosphorus (TDP), soluble reactive phosphorus (SRP), and dissolved organic phosphorus (DOP) in the culture medium at the end of incubation. Additional parameters include cell concentrations from cultures, methane produced by the cultures during the experiment, and rates of C-P lyase activity (CLA). TDP, SRP, and DOP were measured using standard colorimetric methods. Cell concentrations were determined by flow cytometry. Methane concentrations and δ¹³C values were measured with a Picarro gas analyzer, and CLA was quantified using a fluorescently labeled phosphonate probe. These data demonstrate that C-P lyase activity is regulated by dissolved phosphate concentrations and that S. frequens upregulates C-P lyase genes when phosphate concentrations drop below 90 nM. The cultures were grown by Dr. Oscar Sosa, and the samples were analyzed by Dr. Benjamin Granzow.