Drug-susceptibility and resistance indices (RI) of the drug-susceptible LEM3049 isolate for the antileishmanial reference drugs in the different macrophage cell types.

The 50% inhibitory concentration (IC50) of LEM3049 and the RI against pentavalent and trivalent antimonials (SbV and SbIII), miltefosine (MIL), paromomycin (PMM) and amphotericin B (AmB) were determined in primary peritoneal Swiss mouse macrophages (PECs), BALB/c bone-marrow derived macrophages (BMMφ), human blood mononuclear cell-(PBMC) derived macrophages and the commercially available THP-1 and J774 cell lines (not done for RAW cells). Values are expressed as the average IC50 ± SEM and are the result of two independent tests run in duplicate.