Lamin B1 overexpression alters chromatin organization and gene expression (Cut&Run-seq)

Overexpression of Lamin B1 leads to the redistribution of H3K9me3 heterochromatin away from the nuclear periphery and into heterochromatic DNA foci within the nucleoplasm. These changes in chromatin organization correlate with misregulated gene expression (mRNA-sequencing), but do not correlate with altered H3K9me3 deposition across the genome (Cut&Run). Overall design: Cut&Run for H3K9me3 in hTERT RPE-1 stable cell lines overexpressing mCherry or mCherry-LmnB1. Samples include two clonal cell lines for each overexpression construct (mCherry_3, mCherry_4, LmnB1_6, LmnB1_16) and two biological replicates per cell line.