ATF2 and ATF7 mediation of intestinal repair in murine colitis model. ATF2 and ATF7 mediation of intestinal repair in murine colitis model

AP1 transcription factor family members: activating transcription factor 2 (ATF2) and activating transcription factor 7 (ATF7) have highly redundant functions due to homology and hence overlapping DNA-binding sites. The role of these transcription factors in intestinal epithelial homeostasis and repair has not been critically examined. Here, by using an intestine-specific deletion of ATF2 combined with body wide deletion of ATF7 in mice we assess the role of these proteins during intestinal homeostasis and repair after inflammation. Overall design: 12h treatment of WT and ATF2 KO/ATF7 fl/fl colon organoids with 50ng/ml of recombinant mouse TNF-alpha