Wild-type, Foxa2-/-, and Nkx2.2-/- mESCs at different time points of differentiation

We report gene-expression profiling of different mouse embryonic stem cells (mESCs) mutant cell lines differentiating to neuronal lineages upon treatment with Retinoic Acid (RA) and Smoothened agonist (SAG). We generated genome-wide expression profiles of embryonic stem cells and neural progenitors at different differentiating time-points for different mutant cell lines and WT control mESCs. We find NKX2.2 and FOXA2 are key components of an intrinsic timer in neural stem cells founded on a negative feedback network that regulates the duration of motor neuron neurogenesis. mRNA expression profiles of wild-type, Foxa2-/-, and Nkx2.2-/- mESCs at different time points of differentiation.