RNA-seq of injured mouse hearts depleted for myofibroblast Yap and Wwtr1

A myofibroblast specific tamoxifen inducible Cre was used to assess the role of Yap and Wwtr1 in the mouse heart after myocardial infarction Overall design: We implemented a Cre-lox system whereby inducible Cre expressed under the myofibroblast specific Periostin promoter is crossed with Yap and Wwtr1 floxed animals, facilitating inducible depletion of Yap (both alleles) and Wwtr1 (one allele). Mice were siblings with floxed Yap/Wwtr1 alleles and either a copy of MerCreMer under the endogenous periostin promote or wildtype Periostin. Permenant ligation of the left anterior descending artery was used to produce a surgical myocardial infarction. Mice were administered tamoxifen citrate diet ad libitum immediately after surgery to promote excession of myofibroblast yap and left ventricles were collected 4 days post injury.