Expression data from mammary gland during pregnancy and lactation of CBA/CaH, QSi5 and Advanced Intercross Line (AIL; CBA/CaH X QSi5, the 14th generation)

Previously we have shown significant differences in lactation performance, mammary gland histology and expression profiles of mammary transcriptome during peak-lactation (lactation day 9; L9) between the ordinary CBA/CaH (CBA) and the superior QSi5 strains of mice. In the present study, we compared mammary gland histology between CBA and QSi5 at mid-pregnancy (pregnancy day 12; P12). We assessed lactation performance during the first 8 days of lactation of the 13th - 14th generation of the Advanced Intercross Line (AIL) (CBA X QSi5) mice. We utilized an integrative approach to analyzing mammary microarray expression profiles of CBA and QSi5 at P12 and CBA, AIL and QSi5 at L9. The inguinal mammary glands of CBA/CaH and QSi5 during mid-pregnancy (Pregnancy day 12; P12), and the glands of CBA/CaH, AIL and QSi5 during peak lactation (Lactation day 9; L9) were collected and total RNA was extracted for Affymetrix microarray (mouse genome 430 2) assay此前我们已证实，普通品系CBA/CaH（简称CBA）与优良品系QSi5小鼠在泌乳高峰期（泌乳第9天，L9）的泌乳性能、乳腺组织学特征以及乳腺转录组表达谱上均存在显著差异。本研究中，我们比较了妊娠中期（妊娠第12天，P12）时CBA与QSi5小鼠的乳腺组织学差异；同时评估了高级互交系（Advanced Intercross Line, AIL）（CBA与QSi5杂交）第13~14代小鼠泌乳前8天的泌乳性能。我们采用整合分析方法，对妊娠中期（P12）的CBA与QSi5小鼠，以及泌乳高峰期（L9）的CBA、AIL与QSi5小鼠的乳腺基因芯片表达谱进行了分析。本研究收集了妊娠中期（P12）的CBA/CaH与QSi5小鼠的腹股沟乳腺，以及泌乳高峰期（L9）的CBA、AIL与QSi5小鼠的腹股沟乳腺，随后提取总RNA用于Affymetrix小鼠全基因组430 2基因芯片检测。