Tissue-specific concentrations of floral cyanogenic glycosides

Fresh florets of 11 Australian native Proteaceae species (Buckinghamia celsissima, Grevillea robusta, Hakea bucculenta, Helicia australasica, Hollandaea riparia, Lasjia claudiensis, Lomatia myricoides, Macadamia tetraphylla, Megahertzia amplexicaulis, Neorites kevedianus, and Telopea speciosissima) were collected and dissected to quantify tissue-specific cyanogenic glycoside concentrations measured as evolved cyanide following the methods in Gleadow et al. (2011) [1] and Brinker and Seigler (1989) [2]. The results (concentrations.csv) were plotted as bar plots using R Studio script (concentrations plot.R). The cyanogenic glycoside concentrations were expressed as relative concentrations, where the tissue with the highest concentration was scored 1 for each species, and concentrations in other tissues were expressed relative to that value (relativeconc.csv). Relative concentrations were visualised as heatmap and grouped using hclust model function (heatmap.R). References: 1. Gleadow RM, Bjarnholt N, Jørgensen K, Fox J, Miller RE. Cyanogenic glycosides. In: Narwal SS, Szajdak L, Sampietro DA, editors. Research methods in plant sciences. Soil Allelochemicals. 1. USA: Stadium Press LLC; 2011. p. 283-310. 2. Brinker AM, Seigler DS. Methods for the detection and quantitative determination of cyanide in plant materials. Phytochemical Bulletin. 1989;21(2):24-31.