Involvement of Microglia in RGC Injury Induced by IOP Elevation in a Rat Ex Vivo Model

This dataset supports the findings of the manuscript titled: "Involvement of microglia in retinal ganglion cell injury induced by IOP elevation in a rat ex vivo acute glaucoma model." In this study, we used a rat ex vivo model of acute intraocular pressure (IOP) elevation to investigate the role of microglia in retinal ganglion cell (RGC) injury. Our results demonstrate that pressure-induced activation of the TLR4-NLRP3 inflammasome cascade contributes to microglial proliferation and IL-1β production, leading to RGC apoptosis. Pharmacological depletion of microglia using the CSF-1R inhibitor PLX5622 suppressed this inflammatory pathway and preserved RGCs. The uploaded files include:- Raw and normalized data from flat-mounted immunostaining (RGC and microglia counts), Western blot membranes (protein expression), TUNEL-stained cryosections (apoptosis), and Epon-embedded sections (NFL thickness and NDS evaluation).- Source data used to generate the figures. This dataset is made available to promote transparency and reproducibility in glaucoma research, particularly in studies involving retinal neuroinflammation and microglial modulation.