Effects of dietary eggshell membrane powder on gut microbiota composition in interleukin-10 knockout female mice

Inflammatory bowel disease (IBD) is known to be associated with the gut microbiota. This study aimed to investigate whether eggshell membrane (ESM) ameliorates gut dysbiosis in spontaneous IBD model of interleukin-10 knockout (IL10 KO) mice. Female C57BL/6J wild type (WT) and IL10 KO mice (KO) were fed with an AIN 93G basal diet or an ESM diet (KOE) for 19 weeks. Bacterial DNA was extracted from the cecal contents, and gut microbiota profiles were analyzed using 16S rRNA gene sequencing. Our data showed ESM supplementation improved the imbalanced gut microbiota composition, as indicated by the higher bacterial diversity and richness, the elevated abundance of commensal gut bacteria, and the decreased abundance of potentially harmful bacteria (Enterobacteriaceae) in IL10 KO mice.