IL-10 production in mucosal FOXP3+ CD4+ T cells is most prominent in cells lacking Helios.

(A) Representative plots of gating strategy of CD4+ T cells from nasal mucosa and PBMCs when comparing IL-10 and Helios staining with increasing levels of FOXP3 expression, revealing that IL-10 is preferentially expressed by FOXP3+ cells negative for Helios. (B) Compiled percentages from mucosal samples (n = 6) and PBMC (n = 9) Statistical difference between PBMC and nasal mucosa is indicated, *p<0.01, **p<0.0004. (C) Compiled percentages of IL-10+ memory CD4+ T cell distribution within these subsets from nasal mucosa samples (n = 6) and PBMC (n = 9) reveal that the vast majority of IL-10+ cells are found within the FOXP3-Helios- population.