A validation scale to determine the readiness of environmental DNA assays for routine species monitoring

This data set contains the data files and the R-code accompanying the titled manuscript.

"1_literature database.xlsx" contains citations and basic information on the 327 papers used in the meta-analysis.

"2_validation scale checklist.xlsx" contains the validation scores extracted for 546 targeted environmental DNA assays contained in the literature database and a separate spreadsheet with variable definitions.

"3_validation_scale_R_script.R" and "4_HTML file R script validation scale.html" contain the R script which is the basis for all calculations and figures included in the manuscript.
"5_valscale_basic", "6_checklist_metrics_1.csv", "7_val_var_summary.csv", "8_baseforbubbleplot.csv", "9_checklist_metrics_2.csv", "10_forbox1.csv", and "11_forbox2.csv" contain subsets or transformations of the data contained in "validation scale checklist.xlsx" and are accessed by the R-script, which also contains content descriptions of these files.