Summary statistics for the 22 microsatellite markers in <i>Microbotryum saponariae</i> (N = 97).

Repeat: Motifs and repeat numbers of the microsatellite markers in the individual genome from which the markers were developed. Range: allelic size range in number of base pairs (bp). Multiplex: number indicating to which multiplex group the marker belongs. Multiplex 3 a and b were kept separate during PCR amplification to avoid the amplification interference that occurred when all four markers were amplified together, but they were pooled for genotyping in the end. Dye: forward primers of the selected markers were labelled with the following fluorescent dyes: Yakima Yellow equivalent to VIC, ATTO 550 equivalent to NED, ATTO 565 equivalent to PET. Alleles: number of alleles observed in the sample. HO: observed heterozygosity. HE: expected heterozygosity; Symbols (*) and (+) show significant deficit or excess in heterozygosity compared to Hardy-Weinberg expectations (* P < 0.05; ** P < 0.01; *** P < 0.001). FIS: Fixation index. SE: standard error.