Responses of human MAIT cells to CA7S

To investigate the effect of cholic acid sulfate to MAIT cells, PBMCs were stimulated with 5-OP-RU and CA7S and scRNA sequencing was performed. Inflammatory genes were induced by 5-OP-RU, while wound healing related genes were inducesed by CA7S. This analysis showed that cholic acid sulfate may contributes to maintenance of MAIT cells. PBMCs were unstimulated or stimulated with 5-OP-RU or with CA7S overnight. CD161+MR1-5-OP-RU tetramer+ cells were sorted and analyzed by single-cell TCR- and RNA-seq.