Mouse bone marrow Mesenchymal Stem Cells (MSC) exposed to activated T cells. Mus musculus

To analyze the changes induced in MSC transcriptome by activated T cells, MSC were co-cultured in transwell with anti CD3/CD28 activated T cells for 48 hrs before RNA extraction IFNγ-induced plasticity of mesenchymal stem cells is regulated by STATs through inhibition of mTOR and TGFβ pathways. Vigo T, Procaccini C, Ferrara G, Baranzini S, Oksenberg J, Diaspro A, Kerlero de Rosbo N, Uccella A. Overall design: The expression of MSC co-cultured with activated T cells ( two replicates) were compared to that of naive MSC (3 replicates)