Transcriptomic analysis of murine lung cancer cell lines lacking CIC (Capicua) upon treatment with trametinib

To identify the gene expression changes that depend on the activity of CIC (Capicua), we treated Kras(+/G12V);Trp53(-/-) or Kras(+G12V);Trp53(-/-);Cic(D2-6/D-2-6) lung tumor cells with trametinib to block MEK which will activate the CIC repressor. We predicted that CIC activation in Kras(+/G12V);Trp53(-/-) cell would cause repression of genes controlled by CIC. In contrast, Kras(+G12V);Trp53(-/-);Cic(D2-6/D-2-6) lung tumor cell lines express inactive CIC proteins and treatment with trametinib would not cause repression of CIC target genes.