Probes used for the EMSA experiments with C/EBP.

The weight matrix for the consensus C/EBP alpha/beta binding motif is given on top. The consensus C/EBP alpha/beta binding motif was aligned with the potential C/EBP binding sites identified in the human HSD11B2 promoter and located at position −177, −198, −1985, −4362 bp. “cons C/EBP” and “mut C/EBP” designate 20 to 24-mer oligonucleotides based respectively on the consensus and mutated binding site for C/EBP. Mismatched nucleotides with matrix are underlined. In bold are the nucleotides identical to the consensus sequence and the percentage of match with the consensus sequence is indicated.−177 C/EBP, −198 C/EBP, −1985 C/EBP,−4362 C/EBP indicate the probes harboring the putative binding sites for C/EBP alpha/beta located in the human HSD11B2 promoter.