Cognate T cell interactions affect the DC transcriptome [dataset 4]. Cognate T cell interactions affect the DC transcriptome [dataset 4]

Our data suggest that peptide-presenting DC receive signals from synapse-forming antigen specific CD4+ or CD8+ T cells that alter the DC expression profile. To further test this notion, we hock immunized T cell-engrafted mice with NP harboring CpG and coated with OVA (OVA / CpG NP) or the control antigen BSA (BSA / CpG NP) and isolated NP+ DC for RNAseq. Overall design: NP+ DC were isolated 20 hrs post sub-cutaneous immunization with (OVA/CpG) NP or (BSA/CpG) NP from popliteal and inguinal lymph nodes. (OVA / CpG) or (BSA/CpG) NP+ DC were single-cell sorted into 384w-plates, barcoded and MARS-sequenced.