Internalisation of sCT(8-32)-AF568 and 9E10-AF647 in COS-7 cells stably expressing the hCTRaLeu

Confocal field images of COS-7 stably expressing
hCTRaLeu were incubated with 1 μM sCT(8-32)-AF568 and 1 µg/ml
anti-cMyc
antibody (9E10-AF647) on ice. Cells were extensively washed. Imaged
were acquired every 1 min for 60 min at 37 ̊C. Images are presented as maximum intensity
projections for 9E10-AF647 emission (green), sCT-(8-32)-AF568 (red) emission.
Representative images of 2 independent experiments.

将稳定表达人源降钙素受体α亮氨酸变体（hCTRaLeu）的COS-7细胞，与1 μM鲑鱼降钙素8-32片段标记AF568荧光染料（sCT(8-32)-AF568）及1 μg/ml抗cMyc抗体（9E10-AF647）在冰上共同孵育。随后对细胞进行充分洗涤，于37℃下每1分钟采集一次共聚焦视野图像（confocal field images），总时长60分钟。所有图像均以最大强度投影（maximum intensity projections）形式呈现，其中9E10-AF647发射通道对应绿色荧光，sCT(8-32)-AF568发射通道对应红色荧光。本次展示的为2次独立重复实验的代表性图像。

搜集汇总

背景与挑战

背景概述

该数据集包含COS-7细胞稳定表达hCTRaLeu时，sCT(8-32)-AF568和9E10-AF647的内化过程的共聚焦场图像。实验在37°C下进行60分钟，每分钟获取一次图像，数据代表2次独立实验的结果。数据集由Monash University的Emma Dal Maso、PATRICK SEXTON和Sebastian Furness创建，发布于2017年。