mRNA expression data in epididymal fat tissues derived from mir342-/- and mir342wt/wt mice

mir342-/- and mir342wt/wt C57BL/6J mice were fed with standard diet (STD) and high fat-high sucrose diet (HFHS). We used microarrays to investigate the mRNA expression profile in the epididymal adipose tissues. mir342-/- and mir342wt/wt C57BL/6J mice were housed in cages and maintained on a 12-hour light-dark cycle. The were fed with STD (NMF; Oriental Yeast) and HFHS chow (D12331; Research Diet, New Brunswick, NJ). The mice were sacrificed at 16 weeks of age and epididymal adipose tissues were used for RNA extraction and applied to GeneChip Mouse Gene 2.0 ST Array.本研究以mir342纯合敲除（mir342-/-）与mir342野生型纯合（mir342wt/wt）C57BL/6J小鼠为实验对象，分别喂食标准饲料（STD，standard diet）与高脂高糖饲料（HFHS，high fat-high sucrose diet）。我们采用基因微阵列（microarrays）技术探究附睾脂肪组织中的mRNA表达谱。将两种小鼠分笼饲养，维持12小时光暗循环，其中一组喂食STD饲料（NMF；东方酵母公司），另一组喂食HFHS饲料（D12331；美国新泽西州新不伦瑞克市Research Diet公司）。小鼠饲养至16周龄时实施安乐死，摘取附睾脂肪组织进行RNA提取，随后使用GeneChip小鼠基因2.0 ST阵列（GeneChip Mouse Gene 2.0 ST Array）完成表达谱检测。