Material S1 - Photounbinding of Calmodulin from a Family of CaM Binding Peptides

Figure and text S1: Gel electrophoresis to test for monomeric CaM after quencher dye labeling. Figure and text S2: CaM antibody staining. Figure and text S3: Automated image analysis to quantify photounbinding. Text S4: Calculation of the total incident laser flux (incident energy per unit area). Figure and text S5: No bias by fluorescence quenching. Text S6: control: unspecific binding of CaM. Figure and text S7: Probing photounbinding by rebinding of identically labeled A488-CaM. Figure and table S8: Mathematical correction for low affinity peptides. Figure S9: Log-log plot of the unbinding and rebinding fraction. Figure and text S10: Analysis of photounbinding in fixed cells. Figure S11: No photounbinding for quencher dye labeled CaM. Figure S12: Non-radiative energy transfer to CKII-peptide (Jablonski energy diagrams). Text S13: Fitting statistics for plot. Figure S14: Photounbinding experiments before and after PFA fixation. (1.22 MB PDF)